![]() Another enzyme that is involved in the synthesis of hydrogen sulfide is 3-mercaptopyruvate sulfurtransferase (MPST, EC 2.8.1.2). H 2S is endogenously synthesized from l-cysteine in a reaction catalyzed by cytosol enzymes: cystathionine beta-synthase (CBS, EC 4.2.1.22) and cystathionine gamma-lyase (CTH, EC 4.4.1.1) with the participation of the cofactor-pyridoxal phosphate (vitamin B 6). Hydrogen sulfide (H 2S) has a significant effect on the cardiovascular system function and participates in blood pressure regulation. These changes result in impaired body functions, and consequently lead to death. The molecular aspects of aging include genomic instability, telomere attrition, cellular senescence, and mitochondrial dysfunction. The risk of developing cardiovascular disorders increases with age. One of the main causes of cardiovascular disorders is hypertension, which can contribute to diseases of many organs of the body, including heart, aorta, blood vessels, and kidneys. These findings pave the way for interventional studies evaluating a potential causal relation between liver sulfur metabolism, hypertension and aging.Ĭhronic diseases (including cardiovascular diseases) contribute to 60% of the total number of worldwide deaths. In conclusion, our study shows that arterial hypertension and aging affect hepatic sulfur metabolism and H 2S production in rats. Furthermore, differences between younger and older WKY rats in the expression and/or activity of TST and MPST were present. For example, 16-week-old SHR had significantly higher activity of TST than 16-week-old WKY. The gene expression and activity of enzymes involved in sulfur metabolism differed between WKY and SHR, and between the age groups. ![]() SHR livers from both age groups showed a higher capacity to generate H 2S than livers from WKY. The levels of cysteine, cystine, reduced and oxidized glutathione were measured using RP-HPLC. Livers isolated from 16- and 60-week-old normotensive Wistar Kyoto rats (WKY) and Spontaneously Hypertensive Rats (SHR) were used to evaluate gene expression using RT-PCR, and the activity of enzymes participating in H 2S metabolism, including thiosulfate sulfurtransferase (rhodanese TST), cystathionine gamma-lyase (CTH), and 3-mercaptopyruvate sulfurtransferase (MPST). This study evaluated the effects of hypertension and aging on the hepatic metabolism of sulfur-containing compounds, the activity of the enzymes involved in sulfur homeostasis, and the liver’s ability to generate H 2S. Hydrogen sulfide (H 2S), a gaseous transmitter, contributes significantly to regulating arterial blood pressure and aging processes. ![]() Hypertension and age are key risk factors for cardiovascular morbidity and mortality. ![]()
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